Zinc acetate as a precipitant of unstable haemoglobins.

Zinc acetate as a precipitant of unstable haemoglobins.

Whereas the addition of four moles of zinc acetate to one of normal human haemoglobin at pH 7.4 results in a clear solution, addition to unstable haemoglobin results in precipitate formation. Within certain limits no false-positive results are obtained with methaemoglobin, and with fetal haemoglobin. With excess zinc, normal haemoglobin also precipitates, but this process is reversible and can ...

Separation of haemoglobins on cellulose acetate.

Rapid electrophoresis and quantitation of haemoglobins on cellulose acetate.

A rapid and reproducible electrophoretic method for the separation and quantitation of haemoglobins on cellulose acetate is described. The accuracy of the method and its possible sources of error are discussed. The normal range for haemoglobin A(2) by this method is 1% to 3% of the total haemoglobin concentration. Blood samples from 32 thalassaemic patients showed haemoglobin A(2) values of 3.5...

Reactions involving superoxide and normal and unstable haemoglobins.

Superoxide ions (O2-) oxidized oxyhaemoglobin to methaemoglobin and reduced methaemoglobin to oxyhaemoglobin. The reactions of superoxide and H2O2 with oxyhaemoglobin or methaemoglobin and their inhibition by superoxide dismutase or catalase were used to detect the formation of superoxide or H2O2 on autoxidation of oxyhaemoglobin. The rate of autoxidation was decreased at about 35% in the prese...

Zinc Electrodeposition from Acetate Solutions

he electrodeposition of zinc on iron and steel for protection T against corrosion was first proposed in 1840. The combination of aesthetic appeal and protection thus provided has established bright zinc plating as an important process in modern industry. In today's technology, zinc is preferably deposited from acid-type electrolytes, especially chloride, as an alternative to those based on cyan...